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washing solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher washing solution
    Washing Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/Hanks'+Balanced+Salt+Solution+(1X)%2C+without+calcium%2C+without+magnesium%2C+without+phenol+red/pm41997233-65-7-13
    Average 97 stars, based on 1 article reviews
    washing solution - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Construct:

    Article Title: Topological tracks patterned via 3D printing vascularize murine organ-scale constructs
    Article Snippet: Tissue constructs were then incubated for 24 h with anti-human CD31/PECAM-1 DyLight 550 conjugated antibody (1:100, Novus Clone: JC/70A), anti-mouse TER-119 Alexa Fluor 488 (1:100, Biolegend #116215) conjugated antibody, and 5% dimethyl sulfoxide in fresh blocking solution at 37 ◦ C on an orbital shaker. .. After 24 h, constructs were moved into a wash solution (0.2% Triton X-100, 0.5% 1-thioglycerol in PBS) and incubated on an orbital shaker for 6 h, replacing the wash solution every 2 h. Constructs were then transferred to a clearing enhanced 3D (Ce3D) Microscopy clearing solution with Hoechst 33342 (1:500, Invitrogen #H3570) for 24 h at room temperature. ..

    Incubation:

    Article Title: Topological tracks patterned via 3D printing vascularize murine organ-scale constructs
    Article Snippet: Tissue constructs were then incubated for 24 h with anti-human CD31/PECAM-1 DyLight 550 conjugated antibody (1:100, Novus Clone: JC/70A), anti-mouse TER-119 Alexa Fluor 488 (1:100, Biolegend #116215) conjugated antibody, and 5% dimethyl sulfoxide in fresh blocking solution at 37 ◦ C on an orbital shaker. .. After 24 h, constructs were moved into a wash solution (0.2% Triton X-100, 0.5% 1-thioglycerol in PBS) and incubated on an orbital shaker for 6 h, replacing the wash solution every 2 h. Constructs were then transferred to a clearing enhanced 3D (Ce3D) Microscopy clearing solution with Hoechst 33342 (1:500, Invitrogen #H3570) for 24 h at room temperature. ..

    Article Title: Coupling mathematical modeling with a novel human intestinal stem cell system to understand feedback regulation during planar cell polarity
    Article Snippet: .. After blocking, cells were incubated with either sheep anti-VANGL2 (1:100 dilution, R&D Systems #AF4815) or rabbit anti-FZD6 (1:100 dilution, Antibodies.com #A99895) primary antibody in 3% BSA overnight (16 hours) at 4°C, rinsed three times with IF wash solution (0.1% BSA, 0.2% Triton-X 100, and 0.05% Tween-20 in PBS, 5 minute incubation per rinse), then incubated with donkey anti-sheep Alexa Fluor 488 (1:500, Abcam #ab150177) or donkey anti-rabbit Alexa Fluor 488 (1:500, Abcam #ab150073) secondary antibody and DAPI (200ng/mL final concentration, ThermoFisher #D3571) in 3% BSA for 1 h at room temperature. ..

    Microscopy:

    Article Title: Topological tracks patterned via 3D printing vascularize murine organ-scale constructs
    Article Snippet: Tissue constructs were then incubated for 24 h with anti-human CD31/PECAM-1 DyLight 550 conjugated antibody (1:100, Novus Clone: JC/70A), anti-mouse TER-119 Alexa Fluor 488 (1:100, Biolegend #116215) conjugated antibody, and 5% dimethyl sulfoxide in fresh blocking solution at 37 ◦ C on an orbital shaker. .. After 24 h, constructs were moved into a wash solution (0.2% Triton X-100, 0.5% 1-thioglycerol in PBS) and incubated on an orbital shaker for 6 h, replacing the wash solution every 2 h. Constructs were then transferred to a clearing enhanced 3D (Ce3D) Microscopy clearing solution with Hoechst 33342 (1:500, Invitrogen #H3570) for 24 h at room temperature. ..

    other:

    Article Title: TXNIP mediates LAT1/SLC7A5 endocytosis to limit amino acid uptake in cells entering quiescence
    Article Snippet: For double-staining with LAMP1, cells were simultaneously incubated with rabbit anti-LAMP1 (Cell Signaling, #9091) in a 1:800 dilution in blocking buffer.

    Article Title: TXNIP mediates LAT1/SLC7A5 endocytosis to limit amino acid uptake in cells entering quiescence.
    Article Snippet: At around 80% confluency, 16 μg of the respective plasmids were diluted in 500 μl of OptiMEM (Thermo Fisher Scientific, #31985062) in the presence of 48 μl of LTX- and 16 μl of Plus-reagent for the transfection.

    Article Title: Comprehensive mapping of sensory and sympathetic innervation of the developing kidney
    Article Snippet: Sections were incubated for 30 min in blocking solution (ice-cold PBS solution with 4% donkey serum (Equitech-Bio, SD30-0100), 1% bovine serum albumin (Fisher Scientific, BP9706-100), and 0.25% Triton X-100 (Fisher Scientific, BP151-500).

    Staining:

    Article Title: Transcriptional repression of central spindle factors controls endomitosis in the C. elegans intestine
    Article Snippet: .. DAPI staining was performed using 5 μg/ml DAPI in 1 ml wash solution (10% formamide, 2× SSC) for 60 minutes at 37°C, after which animals were mounted on a slide using ProLong gold antifade mountant (Invitrogen #P36934). .. Imaging was performed on either a Nikon Ti2 microscope equipped with a CSU-X1 spinning disk or a Leica SPE confocal microscope using a 40x objective and 0.5 μm Z-slices.

    Blocking Assay:

    Article Title: Frozen media preserves blastocyst development and quality in cattle embryos produced in vitro: a six-month evaluation.
    Article Snippet: Media preparation represents a critical source of variability in cattle in vitro embryo production (IVP), with batch-to-batch inconsistencies potentially affecting experimental outcomes.. This study evaluated whether cryopreservation of IVP media could maintain embryonic developmental competence while reducing preparation-related variability.. We compared the efficacy of frozen (− 80 C) versus freshly prepared synthetic oviduct fluid (SOF)-based media for producing cattle blastocysts over storage periods of one, three, and six months.

    Article Title: Coupling mathematical modeling with a novel human intestinal stem cell system to understand feedback regulation during planar cell polarity
    Article Snippet: .. After blocking, cells were incubated with either sheep anti-VANGL2 (1:100 dilution, R&D Systems #AF4815) or rabbit anti-FZD6 (1:100 dilution, Antibodies.com #A99895) primary antibody in 3% BSA overnight (16 hours) at 4°C, rinsed three times with IF wash solution (0.1% BSA, 0.2% Triton-X 100, and 0.05% Tween-20 in PBS, 5 minute incubation per rinse), then incubated with donkey anti-sheep Alexa Fluor 488 (1:500, Abcam #ab150177) or donkey anti-rabbit Alexa Fluor 488 (1:500, Abcam #ab150073) secondary antibody and DAPI (200ng/mL final concentration, ThermoFisher #D3571) in 3% BSA for 1 h at room temperature. ..

    Concentration Assay:

    Article Title: Coupling mathematical modeling with a novel human intestinal stem cell system to understand feedback regulation during planar cell polarity
    Article Snippet: .. After blocking, cells were incubated with either sheep anti-VANGL2 (1:100 dilution, R&D Systems #AF4815) or rabbit anti-FZD6 (1:100 dilution, Antibodies.com #A99895) primary antibody in 3% BSA overnight (16 hours) at 4°C, rinsed three times with IF wash solution (0.1% BSA, 0.2% Triton-X 100, and 0.05% Tween-20 in PBS, 5 minute incubation per rinse), then incubated with donkey anti-sheep Alexa Fluor 488 (1:500, Abcam #ab150177) or donkey anti-rabbit Alexa Fluor 488 (1:500, Abcam #ab150073) secondary antibody and DAPI (200ng/mL final concentration, ThermoFisher #D3571) in 3% BSA for 1 h at room temperature. ..



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